CRISPR Guide RNA Designer I developed an open-source, interactive web app to help researchers design and analyze CRISPR guide RNAs (gRNAs) with confidence and ease. Built with Python and Streamlit, it combines biology and AI insights to accelerate genome editing research. ✨ Key Features: 🧬 Identify candidate gRNAs based on customizable PAM sequences, guide lengths, and GC content 🔍 Predict off-target effects using mismatch tolerance against background DNA sequences 🧪 Simulate DNA edits and predict impacts on protein translation in silico 🤖 Generate AI-powered detailed reports with Gemini and OpenAI integrations 📊 Interactive visualization and ranking to simplify guide selection 🛠️ Fully open source and accessible to molecular biologists and bioinformaticians 🚀 This tool empowers scientists to design more reliable CRISPR experiments faster, fostering collaboration and innovation in gene editing.